محصولات - CMT reagent

معرف CMT سدیم لوریل سولفات تست ورم پستان کالیفرنیایی

CMT reagent

Sodium lauryl sulfate

California Mastitis Test

Pharmaceutical composition: Each milliliter of the solution contains 50 mg of sodium lauryl sulfate.

Pharmacological characteristics and mechanism of action: Sodium lauryl sulfate reduces the surface tension and changes the structure and conductance of the cell membrane and nucleus, disturbs the osmotic balance, blocks oxidation, stimulates proteolytic enzymes and increases the viscosity of milk. As a result of adding this solution to the milk, it causes the DNA of the somatic cells of the milk to react with the solution and form a gel, which indicates the presence of subclinical mastitis.

Indications: California mastitis test (CMT) is a fast, accurate and feasible test in livestock for early detection of subclinical mastitis and estimation of somatic cell count (SCC). Somatic cells include white blood cells and mammary epithelial cells that enter milk in response to breast inflammation, and counting these somatic cells is used as an indicator to diagnose mastitis. CMT can be used to take samples from each cartier and also to take samples from milk buckets and milk storage tanks in order to determine the condition of the whole herd in terms of mastitis in addition to diagnosing sick cows.

way of doing CMT:
  • Clean the cartiers with alcohol, throw away the pre-milking milk and then wash one or two milks from each cartier in a special container. Add the same volume of milk inside each part, CMT reagent to the container and then slowly rotate it horizontally to mix the contents well. This process should not take more than 10 seconds. Then check the mixture quickly for gel formation (reaction). The presence of gelatinous or stringy material at the bottom of the dish indicates a high Cartier cell count.
Precautions:
  • When taking samples from the milk bucket and the milk storage tank, before taking the sample, the tank milk should be stirred for at least two minutes so that the test result can be shown correctly.
  • If there is doubt about the test result, the test can be repeated immediately or in the next milking.
  • A high or low value affects the result.
  • In case of contact with eyes, rinse immediately with plenty of water.
  • Keep out of reach of children.
  • Color interpretation is not evident in the test, and the criterion is only gel formation.

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Method of interpretation of observations: